Aladdin Neutralization Buffer (1M Tris-HCl, pH 9.0) is a ready-to-use, high-concentration buffer specifically formulated for the immediate neutralization of low-pH eluents collected during antibody purification processes. This buffer enables rapid pH adjustment to protect sensitive antibodies from acid-induced denaturation, ensuring optimal recovery of biological activity.Key FeaturesReady-to-Use: Pre-adjusted to pH 9.0, requiring no dilution or further preparationRapid Neutralization: Quickly restores physiological pH conditions following acidic elutionHigh Buffering Capacity: 1M concentration ensures effective neutralization of low-pH elution buffersPreserves Antibody Integrity: Prevents prolonged acid exposure that can lead to antibody denaturation and activity lossWide Compatibility: Ideal for use with all acidic elution buffers including Aladdin's IgG Elution Buffer (pH 2.7)ApplicationsImmediate neutralization of antibodies following Protein A/G/L affinity purificationCompatible with both manual and automated purification systemsSuitable for neutralizing eluents from various affinity chromatography methodsIdeal for research-scale and process development applicationsInstructions for UseAdd Neutralization Buffer to collection tubes prior to elution (recommended volume: 1/10 of expected eluent volume)Collect acidic eluate (e.g., from Aladdin IgG Elution Buffer, pH 2.7) directly into the bufferMix gently to ensure complete neutralizationVerify final pH if necessary; the mixture should reach approximately neutral pH (7.0-7.4)NotesFor optimal antibody recovery, neutralize immediately after collectionAdjust neutralization buffer volume proportionally for larger-scale purificationsCustom pH formulations (pH 8.0-9.0) available upon request
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- Citation
| Name | Tris-HCl Antibody Purification Neutralization Buffer (1×) |
|---|---|
| CasNumber | Neutralization Buffer |
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| Synonyms | Neutralization Buffer |
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| SKU | Size | Unit | Price $ | Stock | |
|---|---|---|---|---|---|
| N1456179-500ml | 500 | ml | 50 | 1000 | |
| N1456179-100ml | 100 | ml | 17 | 1000 |
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